Fév 05 2016

Imprimer ce Article

Converting bulk sugars into prebiotics: semi-rational design of a transglucosylase with controlled selectivity


Tom Verhaeghe, Karel De Winter, Magali Berland, Rob De Vreese, Matthias D’hooghe, Bernard Offmann and Tom Desmet


Chem. Commun., 2016, 52, 3687-3689 DOI: 10.1039/C5CC09940D


Despite the growing importance of prebiotics in nutrition and gastroenterology, their structural variety is currently still very limited. The lack of straightforward procedures to gain new products in sufficient amounts often hampers application testing and further development. Although the enzyme sucrose phosphorylase can be used to produce the rare disaccharide kojiobiose (α-1,2-glucobiose) from the bullk sugars sucrose and glucose, the target compound is only a side product that is difficult to isolate. Accordingly, for this biocatalyst to become economically attractive, the formation of other glucobioses should be avoided and therefore we applied semi-rational mutagenesis and low-throughput screening, which resulted in a double mutant (L341I_Q345S) with a selectivity of 95% for kojibiose. That way, an efficient and scalable production process with a yield of 74% could be established, and with a simple yeast treatment and crystallization step over hundred grams of highly pure kojibiose (>99.5%) was obtained.

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